Lambda ZAP-CMV Undigested Vector Kit 5 Preparing a –80°C Bacterial Glycerol Stock 1. In a sterile 50-ml conical tube, inoculate 10 ml of LB broth with the appropriate antibiotic (see Recommended Media) with one colony from the plate. Grow the cells to late log phase. 2. Add 4.5 ml...
Proximity ligation assay was performed using Duolink in situ PLA reagents50on Jurkat cells. Cells on slides were blocked with Duolink Blocking stock followed by the application of two PLA probes in 1 × antibody diluent. The slides were washed in a wash buffer (1 × TBS-T) for ...
content="width=device-width, initial-scale=1.0, minimum-scale=1.0, maximum-scale=1.0, user-scalable=no" /> 解放日报社总编辑此前由上海报业集团党委副书记,解放日报社党委书记、社长陈颂清兼任。公开资料显示,陈颂清1965年10月出生,担任解放日报社总编辑近14年。 上海市委组织部10月25日发布的市管干部任职前...
Soon after, I founded theBitcoin Center NYCin 2013 - the world’s first-ever peer-to-peer cryptocurrency trading floor - initially directly across from the New York Stock Exchange (NYSE). I also am the CEO ofBlockchain Technologies Corp., and the holder of a number of blockchain patents,...