9. Basic Concepts of Tangential Flow Filtration (TFF) 9.1 Introduction Membranes use the principle of barrier separation, differentiating components based on size. Components larger than the membrane pore are retained by the membrane while smaller components pass through the membrane into the permeate....
Principle of Tangential Flow Filtration In TFF, a sample flows parallel to the membrane surface in a cassette. A pump provides positive pressure to recirculate the sample between the cassette and a feed reservoir and drive the continuous separation of particles or macromolecules (feed | retentate) ...