[Method]MethodsforRNAisolationisasfollowing:bacteriumwasheatedwithlysisbuffer(TritonX-100andSDSasthemaincomposition),thenRNAwasextrac-ted,precipitatedandwashed.ThepurityandqualityoftotalRNAwasidentifiedbyagarosegelelectrophoresisandnucleicaciddetectionin-strument.[Result]ThemethodisefficientforpureRNAisolationwithout...
Messenger ribonucleic acid (mRNA) represents about 5% of the total cell RNA. It is located in both the nucleus and the cytoplasm. The molecular mass and base composition of mRNA is highly variable. The physiological role of mRNA is the transmission of the genetic information from nuclear DNA ...
we must now understand their detailed composition and dynamics. Methods such as RNA interactome capture (RIC) have begun to address this need. However, limitations of RIC have been reported. Here we describe enhanced RNA interactome capture (eRIC), a method based on the use of ...
To characterize the composition of the hESC interactome, we compiled a catalog of previously annotated RBPs (2,236), DBPs (2,682), and putative DRBPs (478) (Data S3). A comparison of the hESC interactome with this catalog shows that 65% of interactome proteins were classified as “RNA...
A systematic interrogation of male germ cells is key to complete understanding of molecular mechanisms governing spermatogenesis and the development of new strategies for infertility therapies and male contraception. Here we develop an approach to purify
2a). The different composition of TSCs suggests that TSCs possess multiple differentiation potentials. Based on this prediction, three new differentiation lineages related to TSCs were proposed by trajectory and RNA velocity analysis. MSCs differentiate into TSCs. TSCs can further differentiate into ...
Provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit.
220 ml lysis buffer for use with the RNeasy Plus Micro Kit, RNeasy Plus Mini Kit, RNeasy Plus 96 Kit, AllPrep DNA/RNA Micro Kit, and AllPrep DNA/RNA Mini Kit Product Resources FAQs (1) Sort options What is the composition of Buffer RLT plus?
Cells were harvested and incubated in lysis buffer (Beyotime Biotechnology, Shanghai, China) containing protease inhibitor on ice for more than 30 min, a Cell lysate was centrifuged for 15 min at 13,000 rpm at 4 °C, and the supernatant was measured with a bicinchoninic acid protein assay ...
The heterogeneous composition of cellular transcriptomes poses a major challenge for detecting weakly expressed RNA classes, as they can be obscured by abundant RNAs. Although biochemical protocols can enrich or deplete specified RNAs, they are time-cons