1. Add an equal volume of gel loading buffer to the probe or, if the probe has been precipitated, resuspend direct in gel loading buffer. 2. Heat at 95°C for 3-5 minutes to denature any secondary structure, then place on ...
2. Loading and running of gel a. Add an equal volume of gel loading buffer to the probe or, if the probe has been precipitated, resuspend direct in gel loading buffer. b. Heat at 95°C for 3-5 minutes to denature any ...
Reporter: Irina Robu, PhD The CRISPR-Cas9 gene editing system has been welcomed as a hopeful solution to a range of genetic diseases, but the expertise has proven hard to deliver into cells. One plan is to open the cell membrane using an electric shock, but that can accidentally kill the ...
Cells were lysed on ice for 30 min in immunoprecipitation buffer (1% NP-40, 50 mM Tris-HCl, 500 mM NaCl and 5 mM EDTA) containing protease inhibitor cocktail. Protein concentration was determined by the BCA assay (Thermos). Cell lysates (800 μg) were incubated with 2 μg...
Therapeutic mRNAs and vaccines are being developed for a broad range of human diseases, including COVID-19. However, their optimization is hindered by mRNA instability and inefficient protein expression. Here, we describe design principles that overcome
For nutrient treatments, plants were grown in hydroponic cultures using the International Rice Research Institute (IRRI) liquid culture medium recipe (IRRI nutrient solution)38. tEexnctewptafsobramseoddoifnietdhNatHof4NthOe3I,RaRll Innuutrtireinentst were kept solution, at the which same ...
Rice tillering has an important influence on grain yield, and is promoted by nitrogen (N) fertilizer. Several genes controlling rice tillering, which are regulated by poor N supply, have been identified. However, the molecular mechanism associated with t
After centrifugation (47,000 rpm, 45 min, 4 °C, Beckman Coulter SW55 Ti catalog #342194), 500 μL fractions were collected from the bottom of the gradient and lysed, while the remaining pellet was lysed directly in protein lysis buffer (recipe from Cell Signaling Technology, Danvers, MA,...