核酸类:DEPC处理水、MOPS电泳缓冲液、RNA凝胶加样缓冲液(5×)、碱性凝胶加样缓冲液(6×)、50×TAE、5×TBE、6×DNA Loading Buffer、CTAB抽提液、乙酸钠 (3mol/L,pH5.2)、盐酸胍溶液、核酸助沉剂、Denhardt's溶液(50×)、等。 蛋白类:一抗稀释液、Western抗体洗脱液、定影液、显影液、DAB辣根过氧化物酶显...
(5)点离,置于磁力架上,吸去上清,加入500μl rip wash buffer洗两次; [0136] (6)弃上清,加入500μl rip wash buffer,混匀后冰上放置备用; [0137] (7)配制ip buffer,组分如下表: [0138] 表3 ip buffer [0139][0140] (8)取出-80℃保存的rip lysis buffer裂解产物,迅速融化,14000rpm 4℃离心10min;...
Prestained DL5000 DNA Marker(含5×DNA Loading buffer with GelRed)分子量标准 CXCL16 ELISA Kit pET-28a(+) Seamless Cloning Kit(线性化质粒) MK ELISA Kit pBM30 Toposmart Cloning Kit(TOPO克隆载体 原核表达) PDGF-BB ELISA Kit pBM21快速克隆试剂盒(克隆载体 含自杀基因) ...
G4 mt RNA: (UGAGUG)4 Electrophoretic mobility shift assay EMSA was carried out as previously described29. Briefly, PRC2 dilutions and Cy5-H2A labeled mononucleosomes (final concentration 5 nM) were incubated at 4 °C for 30 min in binding buffer (50 mM Tris–HCl, pH 7.5 at...
6)最后取1.5μl rna样品与9μl无核酸酶水及2μl 6x loading buffer混匀,利用1%非变性琼脂糖凝胶电泳分析纯度/完整性/大小,取5μl进行rplc分析mrna纯度,取纯化mrna各5μg电转2*10^6293t细胞,置于37℃5%co2培养箱培养16h,收集细胞100μl 2.5%sds在冰上裂解细胞,13000g离心5min,上清利用elisa检测s蛋白。
点样及电泳:1ul6×Loading Buffer 和5ul PCR产物,混匀后点入凝胶孔中,100v,电泳40min,凝胶成像观测电泳结果。 (这是1.5%的凝胶,配制1%的就可以了,100ml 1×TAE 加1g琼脂。一般用不了100ml,配制20或40ml就够了,称0.2或0.4g琼脂,Goldview也要相应减少。TAE可以买50×TAE,稀释即可。做胶时一定要戴手套,而...
Finally, the cells were washed and then resuspending in staining buffer and analyzing by the BD FACSDiva software. The experiments were repeated for three times 2.9. Statistical analysis The sequencing data analysis was performed as we mentioned above. The data for validation test was collected and...
loading buffer,水浴锅内100℃加热10min,进一步进行十二烷基磺酸钠 ‑ 聚丙烯酰胺凝胶电泳电泳,聚偏氟乙烯膜转膜后5%脱脂奶粉溶液室温封闭1h,洗涤缓冲液洗膜3次,5min/次。 [0086] 2.7抗体孵育及显影观察 [0087] 加入一抗黏连蛋白(fibronectin,1∶5000),一型胶原(collagen i,1∶5 00)和内参(gapdh,1∶10 000...
Tissue sections were deparaffinized, rehydrated, and heat-induced antigen retrieval was performed in an autoclave in 10 mM citrate buffer (pH 6.0) for 5 min. Endogenous peroxidase activity was blocked with 0.3% hydrogen peroxide for 15 min. Sections were incubated with anti-AEG-1 at ...
Red Loading Buffer Pack #B7709S 8 ml-NEB酶试剂 New England Biolabs STCF-hu/mo-Super TCF Cell Line,细胞系(human HEK293 cells)-重组蛋白 DS16570511 食用油标准物质 ART 加长型吸头3512-HR-赛默飞官网代理商 (±)-Terpinen-4-ol;4-松油烯醇 胰蛋白酶,重组体,溶液(人、猪)蛋白研究-wako...