[7] Takahashi K,Migita O,Sasaki A,et al. Amplicon sequencing-based noninvasive fetal genotyping for RHD-positive D antigen- negative alleles[J].Clin Chem,2019,65(10):1307-1316.DOI:10.1373/clinchem.2019.307074. [8] 周一炎, ...
Amplicon sequencing-based noninvasive fetal genotyping for RHD-positive D antigen- negative alleles[J]. Clin Chem, 2019,65(10):1307-1316. doi: 10.1373/clinchem.2019.307074 . 返回引文位置Google Scholar 百度学术 万方数据 [8] 周一炎,秦建江. PCR-SSP RHD基因定型方法的初步应用[J]. 中国现代...
The antigens of the Rh system are encoded by RHD and RHCE, a pair of paralogous genes on chromosome 1. These genes each have 10 exons and share 94% sequence identity. They are very unusual for closely linked paralogous genes as they are in opposite orientation (5′RHD3′-3′RHCE5′...
Methods ABO blood group and RhD antigen were detected by the standard serological methods,RhD antigen negative confirmation and antibody screening were tested by indirect antiglobulin test. Asia Del(RHD*01EL.01 )was detected by PCR-SSP. Results Among 596 pregnant women,70 were confirmed to be...
BACKGROUND: Noninvasive genotyping of fetal RHD (Rh blood group, D antigen) can prevent the unnecessary administration of prophylactic anti-D to women carrying RHD-negative fetuses. We evaluated laboratory methods for such genotyping.Sillence, Kelly A....
全世界范围内,在抗D免疫球蛋白面世之前,Rh阴性女性第2次分娩的婴儿发生RhD同种免疫的比例为16%,RhD相关胎儿和新生儿溶血性疾病是胎儿和新生儿发病和死亡的重要原因,其监测手段主要是胎儿超声监测大脑中动脉峰值血流速度,而治疗手段主要是胎儿宫内输血。...
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2 使用抗-D免疫球蛋白的临床指征 孕妇怀有RhD+胎儿 是否产生抗-D抗体,是否发生HDFN, 首先取决于胎儿红 细胞进入母体循环的时间,数量,概率,以及母体对胎儿 红细胞的免疫反应性, 因此检测胎-母出血量是诊断,预防 RhD HDFN的基础.妊娠过程中,胎儿红细胞通过胎盘进 入母亲体内称为胎母出血(fetal-to-maternal ...
[8] SONNEVELD M E,KOELEWIJN J,DE HAAS M,et al.Antigen specificity determines anti-red blood cell IgG-Fc alloantibody glycosylation and thereby severity of haemolytic disease of the fetus and newborn[J].Br J Haematol,2017,1...
在已知的43个人类红细胞血型系统中,Rh血型系统最为复杂,且可以导致新生儿溶血病(hemolytic disease of fetal and newborn,HDFN)和溶血性输血反应,临床意义仅次于ABO血型系统。其中RhD抗原免疫原性最强,相应的抗D是导致严重HDFN的主要抗体[1]。临床上对于...