In conclusion, in this paper, we have shown that DSF, a FDA-approved aldehyde dehydrogenase inhibitor used as a treatment for chronic alcoholism, and some structural analogues are PHGDH inhibitors. Through biochemical and mass spectrometric experiments, we detailed the mechanism-of-inhibition of PHGDH...
NCT-503 is a small molecular inhibitor specific for PHGDH inhibition. We determined the GI50 of NCT-503 in the MHCC97L cell line (Fig.5a) and found that treatment of NCT-503 significantly reduced the relative ratio of NADPH/NADP+ in cells (Fig.5b). To test whether PHGDH inhibitor could ...