mCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta-barrel (3).mCherry can be used as a long-wavelength hetero-FRET (fluorescence resonance ...
but has extended pi-electron conjugation resulting in red-shifted absorbance and emission (3). mCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta...
Fluorescent proteins are powerful reporters in biology, but most require O2 for chromophore maturation, making them inherently difficult to use in anaerobic bacteria. Clostridium difficile, a strict anaerobe with a genomic GC content of ... EM Ransom,CD Ellermeier,DS Weiss,... - 《Applied & Env...
The binding of LaM2 or LaM4 did not significantly change the chromophore environment of mCherry, which is important for fluorescence quantification assays, while several GFP Nbs significantly altered the fluorescence. Mutation of the residues of the LaM2 or LaM4 binding interface to mCherry ...
Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived fromDiscosoma sp...
[8]Lin J Y ,Sann S B ,Zhou K ,et al. Optogenetic inhibition of 学方法证实ChR2-mCherry 特异表达在谷氨酸能神经 synaptic release with chromophore-assisted light inactivation , ChR2 元上 电生理方法进一步证实 特异表达在谷氨 (CALI)[J]. Neuron ,2013 ,79 (2):24 1 - 253. , AAV2 / 8 ...
(2). The red chromophore of DsRed has a similar topology to GFP, the green fluorescent protein isolated from the jellyfish Aequorea Victoria, but has extended pi-electron conjugation resulting in red-shifted absorbance and emission (3). mCherry is 236 amino acids (aa) in length with a ...
Moreover, mCherry should be an excellent chromophore for homoFRET with r0 of 5.10 nm for energy transfer between two mCherry moieties. Finally, mCherry exhibits higher r0 values than does dsRed. These characteristics, combined with its rapid folding and excellent spectral properties, suggest that...
A discrepancy was observed between experimental and theoretical results, but this could be explained by the chromophore model extracted from the available X-ray diffraction data. While eGFP showed a larger dynamic experimental response (u03b2HRS) due to the highest resonance enhancement, we measured ...
Moreover, mCherry should be an excellent chromophore for homo-FRET with an r0 of 5.10 nm for energy transfer between two mCherry moieties. Finally, mCherry exhibits higher r0 values than does DsRed. These characteristics, combined with mCherry's rapid folding and excellent spectral ...