Helios (Clone 22F6) BioLegend Cat# 137229, RRID:AB_2561639 LAP/IL-10 (Clone JES5-16E3) BioLegend Cat# 505008, RRID:AB_315362 TGFβ (Clone TW7-16B4) BioLegend Cat# 141406, RRID:AB_10898159 ICOS (Clone 15F9) BioLegend Cat# 107708, RRID:AB_313337 GITR (Clone DTA-1) BioLegend Ca...
While the currently approved vaccines induce systemic immune responses, they probably do not evoke mucosal immunity in form of mucosal, secretory immunoglobulin A (IgA) or tissue-resident memory T cells (TRM). Secretory, polymeric IgA can neutralize incoming viral particles at the mucosal surface bef...
The top gene sets reflecting this discordance among the PDE4 inhibitors belonged to the cytoplasmic ribosome, peptide chain elongation, ribosome, protein complex disassembly, and viral infectious cycle biogroups (Table 2; Supplementary Table 3). Therefore, while apremilast gene regulation was focused ...
Real-time quantitative RT-PCR (qRT-PCR) was performed on a CFX96 Real-Time System (Bio Rad) using the HiScript II One Step qRT-PCR Probe Kit (Vazyme) targeting the NiV nucleocapsid (N) gene. Primers and probes used were: forward primer (5’-AACATCAGCAGGAAGGCAAGA-3’), reverse ...
For nearly two decades, the federal government has committed to biopreparedness and made significant progress toward enabling our country to withstand and respond to biological attacks and pandemics. Funding for some of these programs has declined over time, but the new federal budget released last ...
The integrin α4β7 selectively regulates lymphocyte trafficking and adhesion in the gut and gut-associated lymphoid tissue (GALT). Here, we describe unexpected involvement of the tyrosine phosphatase Shp1 and the B cell lectin CD22 (Siglec-2) in the reg
Bristol Myers Squibb, Celgene, AstraZeneca, Illumina, Genentech, Roche–Ventana, GRAIL, Medicxi, Metabomed, Bicycle Therapeutics and the Sarah Cannon Research Institute; has stock options in ApoGen Biotechnologies, Epic Bioscience and GRAIL; and has stock options in and is a cofounder of Achilles...
of Tizona, Werewolf, F-Star, Astellas/Potenza, BMS, Oncorus, Innovent Bio, Kronos Bio and G1 Therapeutics; has grants from BMS, Astellas/Potenza, Tizona and TTMS; patents licensed and royalties from Astellas/Potenza, Tizona and BMS; and stock from TTMS, Tizona, Potenza, Oncorus and Werewolf...
Anti-Mouse CD45.2 Percp5.5 Clone 104 Biolegend Cat:# 109828RRID: AB_893350 Anti-Mouse CD45.2 Bv785 Clone 104 Biolegend Cat:# 109839RRID: AB_2562604 Anti-Mouse CD24 BUV496 Clone M1/69 BD Cat:# 612953; RRID: AB_2870229 Anti-Mouse CD45.1 APCFire-750 Clone A20 Biolegend Cat:# 110752...
Anti-human FcγRIIIA/B antibody (3BioCB16) Thermo Fisher Scientific Cat#25-0168-42; RRID: AB_10714839 Anti-human IgA1/2 antibody (G20-359) BD Biosciences Cat#555884; RRID: AB_396196 Anti-human IgG antibody (HP6017) Biolegend Cat#409304; RRID: AB_10895907 Anti-GFP antibody Abcam ...