此外,CRISPR-Cas13对RNA翻译靶向的影响尚未被探讨。 近日,来自约翰霍普金斯大学公共卫生学院Jiou Wang教授团队的刘宏河博士及其他合作人员在Journal of Clinical Investigation发表题为CRISPR–Cas13d targeting suppresses repeat-associated non...
Jasna BrcicJanez Plavec化学科学与工程前沿:英文版Brčić J, Plavec J. G-quadruplex formation of oligonucleotides containing ALS and FTD related GGGGCC repeat. Frontiers of Chemical Science and Engineering, 2016, 10(2): 222-237
约翰霍普金斯大学王继鸥及其研究团队长期致力于研究ALS/FTD的分子病理机制,对多种遗传型ALS/FTD做了大量深入研究。2023年2月22日,该研究团队在Neuron杂志发表题为DNA-initiated epigenetic cascades driven by C9orf72 hexanucleotide repea...
Pura repaired expanded hexa nucleotide GGGGCC repeat non-coding RNA-caused neuronal toxicity Master Candidate: Zhang Yu Tutor: Associated Professor Li HongLian Division of Histology & Embryology,Department of Anatomy Tongji Medical College,Huazhong University of Science &Technology Wuhan 430030.Hubei,China...
the role of this repeat in C9orf72 gene activity, protein binding, transcript foci formation, and translation of the C9orf72 product, including the noncanonical repeat-associated non-ATG translation (RAN translation) into pathologic dipeptide repeats, as well as any oligonucleotide repeat-based ...
In C9+ FTD, PAF1 and LEO1 are upregulated and their expression positively correlates with the expression of repeat-containing C9orf72 transcripts. These data indicate that PAF1C activity is an important factor for transcription of the long, toxic repeat in C9+ FTD....
We analyzed the GGGGCC repeat expansions of C9orf72 gene in patients with SCA3/MJD from mainland China to determine whether the C9orf72 gene plays a role in the pathogenesis of SCA3/MJD. In our study, there were no pathogenic repeats (>30 repeats) detected in either the patients or ...
然而其具体的发病机制至今不明。已有研究发现GGGGCC六核苷酸重复扩增抑制下游基因转录,且GGGGCC六核苷酸重复扩增非编码RNA (r(GGGGCC)n)可导致神经退行性变。本研究的目的是进一步探讨GGGGCC六核苷酸重复扩增突变对神经元的损伤作用及其可能的机制。我们构建野生型质粒pEGFP-(GGGGCC)3(3个六核苷酸重复片段)和突变型质粒...
An intronic GGGGCC-hexanucleotide repeat expansion in C9ORF72 was recently identified as a major cause of amyotrophic lateral sclerosis and frontotemporal dementia. Some amyotrophic lateral sclerosis patients have signs of parkinsonism, and many parkinsonism patients develop dementia. In this study we exam...
To ascertain whether Lilli also regulates the expression of G4C2repeat RNA, we used a fly model we generated previously in which 5 or 160 copies of G4C2repeats are flanked by human intronic and exonic sequences (Fig.5a)40. Partial loss of Lilli activity decreased the mRNA level of the...