资源名称加德纳菌Gardnerella│vaginalis 种属:Gardnerella│vaginalis 提供形式:冻干物 安全等级:2 模式菌株:yes 培养方法 培养基:865 生长条件:37℃ 存储条件:真空冷冻干燥法 菌种培养: ⒈ 孢子制备 ⑴ 放线菌孢子的制备 一般采用琼脂斜面培养基,培养基中含有一些适合产孢子的营养成分,如麸皮、豌豆浸汁、蛋白胨和一...
5、PCR检测法：(1)根据Belkum等报道加德纳菌位于IIS-23srRNA基因区为特异性引物序列，扩增片段：433bp。引物15′-TTTCGTGGAGGGTTCGATTCTGG-3′引物2 5′-TACA-AGCTGATAGGACGCG-3′由上海生物工程公司合成。(2)临床标本模板DNA的制备：将宫颈分泌物500μl生理盐水中，12 000 r/min离心10 min，弃上清液，加...
Culture and PCR Cultures of Gardnerella vaginalis are not useful for BV diagnosis, as up to 50% of healthy women have positive cultures due to low numbers of G. vaginalis in the vagina without any sign of BV. However, when no wet mounts or Gram stains are available and a clinical diagnos...
The article presents a study on the prevalence of Gardnerella vaginalis in male sexual partners of women with and without bacterial vaginosis (BV) using polymerase chain reaction (PCR). It states that women attending the Jefferson Country Department of Health sexually transmitted disease (STD) Clinic...
1)Gardnerella vaginalis加德纳菌 1.Analysis of DNA detection results of Gardnerella vaginalis and Ureaplasma urealyticum isolated from the abnormal pregnant women;异常妊娠者阴道加德纳菌和解脲脲原体DNA检测结果分析 英文短句/例句 1.The Research of Biotypes and Drug Susceptibility Test of Gardnerella Vaginosis...
8.The Detection of Gardnerella Vaginalis by Real-time Fluorescent Quantitative Polymerase Chain Reaction and Its Clinical Application实时荧光定量PCR检测阴道加德纳菌的方法与应用 9.Research on Prevention of Gardnerella Vaginalis of fox in Large-scale fox Farm;大型养狐场狐阴道加德纳氏菌病的防制研究 10.Det...
(PCR) assays for 12 bacterial species: Lactobacillus iners, L crispatus, L jensenii, Gardnerella vaginalis, Atopobium vaginae, Megasphaera spp, Prevotella... D Leckel - 《Beginning Teachers》 被引量: 0发表: 1989年 2 Vaginal flora in women from Greenland assessed by 3 microscopy and quantita...
Overgrowth of Gardnerella vaginalis causes an imbalance in vaginal microecology. The pathogenicity of G. vaginalis is directly regulated by the cAMP receptor protein (CRP). In this study, we resolve the crystal structure of CRPGv at a resolution of 2.22
The clinical significance, Gram stain reaction, and genus affiliation of Gardnerella vaginalis have been controversial since Gardner and Dukes described th... Catlin,B W - 《Clinical Microbiology Reviews》 被引量: 295发表: 1992年 Detection of bacterial vaginosis-related organisms by real-time PCR fo...