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开展苹果LIM家族的生物信息学及表达分析研究,可为苹果LIM蛋白功能的深入研究,以及今后培育无锈苹果新种质奠定理论基础。【前人研究进展】真核生物的LIM蛋白分子结构中往往包含1个或多个锌指结构,并且都含有高度保守的LIM结构域,通常由50余个氨基酸残基构成,其序列为:CX2CX16-23HX2CX2CX2CX16-21CX2-3(C/H/D)...
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000 cells in 8 μm trans-wells. 0.2% FBS and TNF-α were added in lower chamber to activate the MSCs migration. After 6 hours, viable migrated cells were detected via CellTiter-Glo® Luminescent reagents according to manufacturer's manual. The data revealed that capability to respond to ...