The FPbase spectra viewer enables the comparison of hundreds of FPs and organic dyes with thousands of filters, light sources, and detectors from many vendors. Shown here is a typical use case: the calculation of emission collection efficiency for multiple FP–emission filter pairs. Spectra are (...
DatabaseFluorescent proteinsSpectraVisualizationFPbase is a database of fluorescent proteins and their characteristics and a set of online tools that facilitate searching the database and performing experiments with fluorescent probes. This chapter serves as a general reference for using and searching the ...
This chapter serves as a general reference for using and searching the database and a guide to some of the more commonly used tools including the spectra viewer, custom microscope pages, and FRET calculator. Important caveats when evaluating the data are also discussed.关键词:...