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The protein concentration of the resulting supernatant was measured with BCA kit according to the manufacturer’s instructions. Trypsin digestion An equal quantity of total protein in each sample was enzymatically digested, and the volume was adjusted to be consistent across the sample set. TCA was ...
通亚汽车CTY5121THBCA车载泵产品介绍 仅选用底盘5000mm轴距,选装有无烟筒装置,ABS系统控制器型号:CM4XL,ABS系统控制器生产企业:长春科密汽车制动有限公司,侧面防护装置所用材料材质为:Q235,侧防护连接方式为:螺栓连接。装料斗最下缘离地高度为:500mm,起到防护作用。
A BCA protein assay was used to determine the protein concentration in the supernatant. Equal amounts of protein (20 µg) were separated via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electrically transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, MA...
Lysine lactylation (Kla) has recently been reported to participate in regulating transcription in human cells. However, the characterization, regulatory mechanism and functional consequence of Kla in prokaryotes remain unclear. Here, we report that YiaC functions as a lysine lactylase and that CobB ...
CELLSBackgroundBladder cancer (BCa) is the fourth most common malignant tumor with a poor prognosis worldwide. Further exploration and research are needed to unmask the underlying roles and molecular mechanisms of circular RNAs. In the current study, our findings showed that circXRN2 suppresses ...
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Tissues or cells were lysed with RIPA lysis buffer containing protease and phosphatase inhibitors for 30 min on ice, centrifuged at 12,000 rpm for 15 min at 4 ℃ and supernatant collected. BCA method was used to. The protein concentration was determined by the BSA method and the final concen...
To participate in dancing skills development program.13. What does the underlined word “stereotype" in paragraphA. A fixed concept.C. A strange idea.14. What do Steph Aiello and Edna Serrano think ofthe RoB. Inspiring. 相关知识点: 试题来源: 解析 【解析】BCA 反馈 收藏 ...