RNA is extracted from cellular material with a reagent that includes heparin, a reducing agent to reduce disulfide bonds, a chelating agent, a buffer, and an alkali metal halide. The reagent does not require the use of organic solvents, and the reagent allows extraction to be performed in a...
2. The extracted samples should avoid repeated freeze-thaw cycles, otherwise it will affect the quantity and quality of RNA extraction.3. Before use, please check if there is any crystallization or precipitation in the Buffer RL. It can be heated at 56 ℃ and re solved. Please add Buffer ...
用途:红细胞裂解液(Red Blood Cell Lysis Buffer,或称 ACK Lysis Buffer)是一种去除红细胞最简便易行 的方法,即用裂解液裂解红细胞,它既不损伤有核细胞又能充分的去除红细胞。裂解液裂解是一种比较 温和的红细胞去除方法,主要用于经酶消化分散的组织细胞的分离纯化,淋巴细胞的分离纯化以及组织 细胞蛋白与核酸提取...
红细胞裂解液(Red Blood Cell Lysis Buffer),也称ACK Lysis Buffer,是一种用于从人或鼠等的血液或组织样品中裂解并去除无细胞核红细胞的溶液。 本裂解液经过优化配方,在裂解红细胞的同时几乎不损伤淋巴细胞(lymphocyte)或其它有细胞核的细胞。 本裂解液的主要有效成分为氯化铵。
Serum-starved cells are treated as desired, washed with ice-cold phosphate-buffered saline [PBS, 10 mMNa2HPO4-HCl (pH 7.3), 1.5 mMKH2PO4, 3 mMKCl, 137 mMNaCl], and lysed in ice-cold NP-40 lysis buffer (400μl for a 10-cm dish). All subsequent steps are performed at 4°. Lysa...
containingTRIzolLS Reagent (Ambion), orlysis buffer(from Macherey-NagelRNAkit) to reduce the risk ofRNA degradation. Volumes of reagent (TRIzol LS or lysis buffer from the kit) were selected based upon the sample:reagent ratio recommended by the manufacturer'sRNA extractionmethod. For example, ...
automated single-cell lysis, RNA extraction, and cDNA synthesis for up to 800 cells in parallel on a single chip. This platform offers lower false positives and less bias than tube-based technologies. However, its major drawbacks include the number of cells (>1000) required for capture and ...
To investigate whether constructing small RNA libraries directly using the cell lysis of single oocyte would cause ligation bias or degradation of small RNAs during cell lysis and heat incubation, 5 to 10 GV stage human oocytes were pooled for RNA extraction and approximately 1 ng of total ...
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Adipose-derived stem cells (ADSCs) have been extensively used in preclinical and clinical trials for treating various diseases. However, the differences between ADSCs from lean individuals (L-ADSCs) and those from obese individuals (O-ADSCs) have not bee