CDH1基因介绍.docx,CDH1基因突变诊断研究进展 摘要: E-钙黏蛋白(cadherin)基因(CDH1基因)外显子突变是目前已知的、导致HDGC等多种肿瘤发病的最重要因素, 筛查其突变情况可以用于指导肿瘤的临床诊治,CDH1基因的其他改变, 如内含子突变、基因甲基化及单核苷酸多态性也可能
MATERIAL AND METHODS: With PCR, denaturing high-performance liquid chromatography (DHPLC) and direct sequencing, we screened all exons of the E-cadherin gene for mutations in 80 Chinese gastric cancer patients, 62 of whom had positive family history, we investigated 80 healthy individuals and ...
2A). Immunohistochemistry showed positive staining for cell CyclinD1, CD10, Caldesmon, and Vimentin in the tumor cells, with some tumor cells also demonstrating positive labeling for MyoD1, CKpan, and Myogenin (Fig. 2B, C, D). Tumor cells were negative for estrogen receptor, smooth muscle ...
This FAQ is provided for informational purposes only and is not intended as medical advice. A clinician’s test selection and interpretation, diagnosis, and patient management decisions should be based on his/her education, clinical expertise, and assessment of the patient. ...
We provided convincing evident for the positive regulation of PI3K and negatively regulation of MAPK on Cdh1 in the maintenance of lung CSC self-renewal and stem cell properties (Figure 4). Further, using the nude mouse xenograft and orthotopic lung transplantation model, characterized the effect ...
aMutation of the Chk1-mediated Cdh1 phosphorylation sites inhibits proliferation. HeLa cells were transfected with histone H2B-GFP and either vector or the indicated Cdh1 constructs. After 24 h, proliferation of the GFP-positive cells was analyzed over time.bMutation of the Chk1-mediated Cdh1 ...
6a, Cdh1 loss induced apoptotic cell death as shown by the dramatic increase in the proportion of active caspase-3-positive cells in the VZ/SVZ. To further confirm the progenitor cell nature of the dying cells in the Cdh1 À / À brain, we next looked for Tbr2—an intermediate ...
A mutation in the MEN factor Tem1 suppresses bub2Δ-induced sister-chromatid segregation [28]. It suggests that precocious activation of the MEN causes mitotic slippage, but the molecular mechanism responsible is largely unknown. Although securin degradation and sister-chromatid separation are normally...
IHC results were evaluated by calculating the percentage of positive nuclei, the percentage of positive cells and the staining concentration. Every assessment was carried out by three separated advanced pathologists utilizing the parallel microscope. Experiments were performed in triplicate. RNA-seq ...
The ARHGAP35 gene encoding p190A RhoGAP (p190A) is significantly altered by both mutation and allelic deletion in human cancer, but the functional implications of such alterations are not known. Here, we demonstrate for the first time that p190A is a tumor suppressor using a xenograft mouse mo...