BMC Genomics (2018) 19:305 Page 3 of 11 TruSeqTM RNA Sample Preparation Kit (Illumina, San Diego, California, USA), and paired end reads of 100 bp were sequenced using the Illumina® HiSeq 2000 plat- form. These procedures were performed by Macrogen (South Korea). Cleaning and ...
After blocking in 5% non-fat milk for 2 h, the membranes were incubated with primary antibodies: FUT4 (1:1000, Proteintech, Wuhan, China), CD44 (1:1000, Abcam, Cambridge, UK), LTL (1:2000, Vector, California, USA), LeY (1:500, Abcam), p-GSK3β (1:500, Affinity Biosciences, ...
Division of Biology, MC 156-29, California Institute of Technology, Pasadena, 91125, California, USA Ali Mortazavi, Brian A Williams, Kenneth McCue, Lorian Schaeffer & Barbara Wold Corresponding author Correspondence to Barbara Wold. Supplementary information Supplementary Text and Figures Supplementary Fi...
Full size table Prior to sequencing, RNA quality was measured for each sample using an Agilent 2100 Bioanalyzer®. One library per sample was prepared using the Illumina® TruSeqTM RNA Sample Preparation Kit (Illumina, San Diego, California, USA), and paired end reads of 100 bp were seque...
(1:2000, Vector, California, USA), LeY (1:500, Abcam), p-GSK3β(1:500, Affinity Biosciences, Cincinnati, OH, USA), GSK3β(1:500, Affinity Biosciences),β-catenin (1:500, Affinity Biosciences), PCNA (1:100, Proteintech) and GAPDH (1:5000, Proteintech) at 4 °C overnight. ...
Prior to sequencing, RNA quality was measured for each sample using an Agilent 2100 Bioanalyzer®. One library per sample was prepared using the Illumina® TruSeqTM RNA Sample Preparation Kit (Illumina, San Diego, California, USA), and paired end reads of 100 bp were sequenced using the ...