Abstract. Background/Aims: In diabetic nephropathy (DN), the current angiotensin-II-blocking pharmacotherapy is frequently failing. For diabetic
The cultures were terminated 2 to 3 weeks after seeding by removal of the culture medium, 3 times flushing with PBS, and fixation in 0.04% glutaraldehyde solution. Before fixation, some cultures were stimulated with recombinant human 2 ng/mL vascular endothelial growth factor (VEGF)19 for 5 to...
Recombinant hCMEC/D3 cells (1×106 cells/ml) transduced with either LXRα shRNA, LXRβ shRNA, or non-targeting shRNA were seeded in 24-well plates in culture medium. RNA was isolated using the TRIzol® method (Life Technologies, Bleiswijk, The Netherlands) and cDNA was synthesized using...
Pichia yeast secretes the target protein into culture medium so that the accumulated protein can be harvested easily by centrifugation, followed by general chromatographic technique. Results showed that the ideal purification was achieved using a combination of affinity chromatography and anion exchange ...
20. Multinucleated giant cells with osteoclast-like features form from the peripheral blood mononuclear layer in patients with carinoma of the breast metastatic to bonedoi:10.1016/8756-3282(88)90058-0AR Morton and NG Testa and TD Allen and J McClure and A Howell and DC AndersonBone...
(Rickettsiaceae). However, recently, more systematic investigations using either culture-dependent or molecular (culture-independent) approaches have been performed to demonstrate that the fly microbiome is far more rich and diverse, as it will be shown below. Although the interactions between the ...
hPBMC isolated from different donors (AD n = 5, non-AD n = 8) were incubated with 50 ng IL-4 or 50 ng IL-4 + 500 ng antagonist as previously described. After 14 days the total numbers of cells were counted in 100 μl of the cell culture. For the in ...
[...] “eradication of poverty, especially extreme poverty” and the “contribution of information and communication technologies to the development of education, science and culture and the construction of a knowledge society”, but also collaboration in fields like science education (in cooperation ...
Cell culture HEK293 cells were cultured in a 6-well plate with low glucose DMEM, with 10% FBS and 1% Pen/Strep. Transfection of pCAG-2A-H2B Venus_mOBRb-HA was carried out using the FuGene® HD transfection reagent (Promega, Madison, WI, USA) as per the kit’s instructions with 1.5...
provides means to derive the clinically relevant parameters blood flow and perfusion from the vessel geometry in microcirculation images. Practical Approach Acquiring blood flow velocities and decorrelation times in vivo was realized using the integrated SDF-LSCI sys- tem as schematically shown in Fig....