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sequence different onthe we length amplificationefficiency,andfinally unpairedsequence a G2677T/A. analyzedsequencecontaining and hemin toconstructa In usedG一4DNAframe addition,we binding moiety chemiluminescent thevisual used DNAzyme platform.Toaccomplish detection,we tocleavethe chainoftheRCA restriction...
For example, the restriction enzyme BamHI, which binds the DNA sequence 5′-GGATCC-3′ inhibits damage at the guanine doublet located within its binding site [28]. BamHI makes extensive hydrogen bonding contacts to the guanines in its restriction site, and these interactions are proposed to ...
Amino acid residues Asn116 and Ser118 of the restriction endonuclease Bam HI make several sequence-specific and water-bridged contacts to the DNA bases. An in vivo selection was used to isolate Bam HI variants at position 116, 118 and 122 which maintained sequence specificity to GGATCC sites....
A total of 252 chromosomes from 126 patients with phenylalanine hydroxylase (PAH) deficiencies were analyzed for both mutant genotypes and restriction frag... M Berthelon,C Caillaud,Franoise Rey,... - 《Human Genetics》 被引量: 46发表: 1991年 Two mutations within the coding sequence of the ph...
If the tobacco DNA was digested with restriction endonucleases which have no target site in HRS60.1, the larger part of DNA homologous to HRS60.1 remained as uncleaved "relic" DNA. These results suggest a tandem arrangement of this DNA repeat unit. Four other clones of tobacco nuclear DNA ...
kDa.17BamHI recognizes the duplex symmetrical sequence 5′-GGATCC-3′ and catalyzes double-stranded cleavage between the guanines in the presence of Mg2+.18After digestion byBamHI, the DNA hybrid was cleaved at a specific site and the electrochemical signal of thionine was decreased or ...
BamHI is a restriction endonuclease that binds as a dimer to the palindromic sequence 5′-GGATCC-3′ and hydrolyzes the phosphodiester linkage between the two guanines in the presence of Mg2+ [20]. The X-ray structure of the BamHI-DNA complex shows that direct hydrogen bonding involved in ...
Different effects of base analog substitutions in BamHI restriction site on recognition by BamHI endonuclease and BamHI methylase. Biochem. Biophys. Res. Commun. 1995; 206 :997–1002.Kang YK, Lee B, Nob MJ, Cho N-Y, Yoo OY. Different effects of base analogue substitutions in BamHI ...
Restriction of the high-molecular-weight concatameric RCA products with ApaI, KpnI, BamHI, PstI, and XhoI will result in ~2.7 kb fragments, which can be eluted from agarose gels using a GeneJet Gel Extraction Kit (Thermo Fisher Scientific). The begomovirus-infected samples can be selected for...