同裂酶| 单字母标示| Pronunciation: 符合省时酶(Time-Saver™)标准,可在 5-15 分钟内完成酶切 可提供高保真(HF®)版本酶(NEB #R3136),随附rCutSmart™缓冲液 随附1 管6X 紫色凝胶上样染料 限制性内切酶酶切位点:G/GATCC 精选视频 Reduce Star Activity with High-Fidelity Restriction Enzymes ...
(DNAzyme). Resuits 1.The2-OMeNAsubstituted of recognitionsequence resistancebehavior.The2-OMeNA a demonstrated site-dependentcleavage aresistance substitutionatthefourth ofBamHIalsoshowed recognitionsequence hadthe G the resistance,but1,C5 BamHI,T4strongestcleavage againstcleavage.For enhancedthe andC6 ...
作者签名:导师签名:日期:曰期:2口b.6.J学位论文数据集中图分类号Q783.1学科分类号350.25论文编号1001020121298密级学位授予单位代码10010学位授予单位名称北京{t y-大学作者姓名**学号2009001298获学位专业名称微生物与生化药学获学位专业代码100705全国工具酶标准化委课题来源研究方向生化与分子生物学员会论文题目限制性...
The inhibition was decreased by preliminary incubation of the enzyme with DNA or competitive inhibitors which were the 5'-phosphoryl deoxydinucleotide subsets of the BamHI recognition sequence. The dinucleotide pdGpdG protected the enzyme most efficiently against the butanedione modification. Dinucleotides ...
Crystallographic studies were also recently carried out on the structure of BamHI bound to its recognition sequence in the presence of Mn2+ and Ca2+ [30••]. (It is to be remembered that BamHI is unique in having three acid groups in the active site [Table 1].) The anomalous differ...
Studies on the mechanism of methylation indicate that both strands of the recognition sequence are modified in a single binding event. The sequence specificity of the methylase is relaxed upon the addition of glycerol in the reaction mixture. In the presence of 30% glycerol the enzyme methylates ...
Classical phenylketonuria is an autosomal recessive disease caused by a deficiency of hepatic phenylalanine hydroxylase (PAH). The abolition of an invariant BamHI site located in the coding sequence of the PAH gene (exon 7) led to the recognition of two new point mutations at codon 272 and 273...
The large difference in hydration changes by both BamHI and EcoRI could be attributed to these dissimilar secondary structural features, as well as the functional differences of the two endonucleases during site-specific DNA recognition. 展开
Alphabetized List of Recognition Sequences Cleavage of Supercoiled DNA Compatible Cohesive Ends and Generation of New Restriction Sites Dam-Dcm and CpG Methylation Frequencies of Restriction Sites Isoelectric Points (pI) for Restriction Enzymes Isoschizomers ...
Experimental results demonstrated that OMeN-substituted recognition sequences influenced the cleavage rates significantly in a position-dependent manner. OMeN substitutions can reduce the cleavage as expected. Surprisingly, OMeN substitutions can also enhance the cleavage rates. The kinetics parameters of V ...