and observed with a microscope Axioplan2 (Carl Zeiss) equipped for epifluorescence microscopy and differential interference contrast optics. Images were collected with a digital Axiocam (Carl Zeiss). Bacterial challenges Adult LL01 and YR2 aphids were injected with approximately 300E. coli(DH5 a) ...
Manual scoring of probes was achieved with a side-view camera mounted on a stereo microscope and The Observer XT software. Automated video tracking was performed with a top view on the arena (275 pixels/mm) and EthoVision XT software (Noldus Information Technology bv, Wageningen, The ...
The number of newborn nymphs (viable, viable defective or non-viable defective) was recorded under a light microscope. Microscopy images and length measurements of newborn nymphs were performed with a Leica MZ FL III stereomicroscope (Leica, Wetzlar, Germany) equipped with an Olympus XC50 color ...
Here we present atomic-resolution crystal structures of polerovirusNRTDs, which define a two-domain architecture with a unique, interdigitated topology. Our structures rationalize phenotypes observed in previous mutagenesis studies and provide new insights into the organization of the RTD on the capsid...