Maintenance, Repair & Operations
Abundance changes were also observed for Ecm29 (0.0011 fold, p-value = 9.5E-5) but its lower level in the 26S relative to the 30S is anticipated given its roles in the assembly of the 20S and 19S species and in proteasome quality control. The higher amount of Blm10 in the 26S (...
The last 192 residues of CaSR C-terminus were replaced by the sequence coding C1KKXX (C1, the coiled-coil sequence of 47 residues at the C-terminus of GABAB1) or C2KKXX (C2, 49 residues of GABAB2 coiled-coil region, followed by the endoplasmic reticulum retention signal KKTN) to ...
G4-Hemin strands (B strand) and guidance template (C strand) were simultaneously mixed in 100 μL TA-Mg2+buffer, annealed at 95 °C for 5 min to form complete CEAS, then cooled to room temperature and stored at 4 °C before use. In addition, there was another assembly mode...
For the LacO-LacI tethering experiments (Figs. 3g and 4d), we used a vector containing CFP–LacI, followed by a previously published multiple cloning site20. The RNAPII-CTD plasmid was cloned via digestion with AsiSI (NEB) and BsiWI (NEB) using the NEBuilder HiFi assembly master mix. The ...
We used this to perform RNAseq (Illumina) and obtain a de novo assembly of the total transcriptome of M. balsamina. A Diamond BLAST search for translated proteins showed significant homology to the Hevamine A-like protein from M. charantia (see Fig. 4B). There are several possibilities why...
Assembly procedure of Zn x Mn1−x Fe2O4/Ag–AgVO3 nano-heterostructures. 2.5 Characterization of synthesized materials Various techniques were used to characterize the recently synthesized photocatalysts, including X-ray diffraction (XRD), vibrating sample magnetometry (VSM), ultraviolet (UV)–visible...
KBTBD4R313PRRand KBTBD4P311PPcan interact with CoREST, but not ubiquitylate or degrade it due to the inactivation of CRL3KBTBD4by MLN4924 (Fig.1D). Of note, KBTBD4WT, KBTBD4R313PRRand KBTBD4P311PPall bound equivalently to Cul3, showing that changes in CRL3 assembly were not ...
Division of Health Aspects of Physical Activity, Department of Exercise Science, Arnold School of Public Health, University of South Carolina, 921 Assembly St., 1st Fl. Suite, RM 131, Columbia, SC, 29208, USA Michael W Beets Corresponding author Correspondence to Jennifer L Huberty. ...
(2002)Mutations altering the cleavage specificity of a homing endonuclease, Nucleic Acids Research30: 3870-3879. Combinatorial assembly is a method where protein subunits form different enzymes can be associated or fused. Arnould et al. (2006)Engineering of large numbers of highly specific homing ...