HMW glutenin nucleotide sequences were acquired from GenBank and aligned using MegAlign software (DNA*, Madison, WI, USA). Primers were designed for each glutenin gene based on nucleotide differences that distinguished each glutenin from the other three. Genome specific primer design for the large...
+-N, but opposite to SBD. EC and SBD gradually decreased from the bank to the inside, but other indicators gradually increased from the riverbank inward. This was mainly due to the gradual decline of terrain, which made the groundwater level shallower in the inside than that in the bank....