mCherry gene is functionally expressed in L. major parasites.Estefania CalvoÁlvarezNestor Adrian GuerreroRaquel ÁlvarezVelillaChristopher Fernández PradaJose María RequenaCarmen PunzónMiguel Ángel LlamasFrancisco J. ArévaloLuis RivasManuel Fresno...
为了达到上述目的,首先需要筛选得到含有 PB 转座子的转基因斑马鱼,我们对 PiggyBac 二元转座 系统的供体质粒 - 转座子载体质粒 5'-PTK-3' 进行改 造,构建了一个无外源启动子,包含 PiggyBac 转座 子的 5'-TR 及 3'-TR, 剪接受体(the mouse engrail- ed-2 gene splicing acceptor,En-2SA),内部核糖体...
mCherry红色荧光蛋白 產品图片 GTX630195 ICC/IF Image mCherry antibody [GT844] detects mCherry protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. ...
Su G, Wang L, Gao G, Wu S, Yang L, Wu M,et al. C23 gene regulates the nucleolin structure and biosynthesis of ribosomes in bovine intraspecific and interspecific somatic cell nuclear transfer embryos. FASEB J. 2021;35:e21993pubmedpublisher ...
图1 iStop技术原理(图片来源:Kuscu, Cem, et al. "CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations." Nature methods 14.7 (2017): 710-712.) 1、设计mcherry基因敲除靶点 为了直观地检测基因敲除效果,我们选择了293V-mcherry细胞株。该细胞稳定整合mcherry红色荧光蛋白,并且是单克...
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entrez_gene genbank_ids mutation namemCherry shRNA_sequence size902 species tags locationC terminal on backbone tagSuperecliptic pHluorin resistance markers : 177 tags : Unknown more info or order: Addgene product webpage company information ...
Anti-mCherry,抗mCherry(Cherry荧光蛋白)山羊多克隆抗体,货号AB0040-200/AB0040-500,应用包括WB, IHC (F), IHC (P), IF, IEM。 Anti-mCherry,AB0040-200/AB0040-500应用示例: 免疫荧光--利用mccherry - rab11a转导的MCR细胞使用抗mCherry抗体处理; ...
Simultaneous expression of a protein of interest and mCherry is made possible by the presence of an encephalomyo-carditis virus internal ribosome entry site (IRES; 2) positioned between the multiple cloning site (MCS) and the mCherry gene. The IRES allows a protein of interest and mCherry to...
Marc 等人在高等植物香 豆中首次构建出诱导性瞬时表达的GFP-MAP4蛋白(Marc J,Granger C L,Brincat J,Fi sher D D, Kao T~H, Mccubbin A G and Cyr R J (1998)A GFP-MAP4reporter gene for visualizing cortical microtubule rearrangements in living epidermal cells. The Plant Cell OnlinelO, 1927-...