For protein lysate preparation, cells were first washed with PBS and lysed with Tris Lysis Buffer (1M tris buffer, pH 6.8, 2M NaCl, 1M MgCl2, 0.5M EDTA, 100nM EGTA, triton-X and glycerol) supplemented with protease inhibitor (Roche, 05892791001) and phosphatase inhibitor (Roche, 4906837001)...
Furthermore, we demonstrate that inactivating GNA13 by targeting its palmitoylation enhanced the sensitivity of GCB-DLBCL to the BCL2 inhibitor. These studies indicate that the loss-of-function mutation of GNA13 is a biomarker for BCL2 inhibitor therapy of GCB-DLBCL and that GNA13 palmitoylation ...
Interdicting GNA12/13 signaling using the specific inhibitor p115-RGS inhibits invasion, migration and prevents distant metastasis in mice [20,25,26]. On the other hand, expression of Gα12-QL (a dominant active mutant of GNA12) in breast and prostate cancer cells has been shown to ...