GFP is an endogenous protein from the jellyfish Aequorea Victoria. It was isolated by Osamu Shimomura in 1962. In 1992, the sequence of GFP was cloned (Douglas Prasher) and Martin Chalfie’s lab expressed the sequence in vivo. Roger Tsien’s lab improved GFP and managed to convert it into...
从维多利亚多管发光水母(Aequorea victoria,下文简称发光水母或水母)中分离的绿色荧光蛋白(Green Fluorescent Protein,GFP),让曾经不可见的蛋白质成为可见,在过去的二十多年里成为生物学家和医学科学家研究细胞内各类生物化学过程的指路星。本文要讲述的就是几位科学家的故事,他们为GFP引发的生物学革命做出了重要贡献。
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. 蛋白标签: N-terminal 6xHis-SUMO-tagged 产品提供形式: Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you ...
is any one of the insertion point in the wild-type mouse hepatitis coronavirus genomic RNA of the inserted fragment in the fragment of a b; a fragment of the sequence Sequence Listing 1 encoded RNA; b is the fragment containing the DNA fragment encoding the green fluorescent protein coding ge...
1、实用文档绿色荧光蛋白(GFP)基因的克隆、表达和粗提取南方医科大学2011预防医学(卫生检验检疫)摘 要目的:研究绿色荧光蛋白(green fluorescent protein , GFP基因在大肠杆菌中的基 因克隆与重组表达,以及对其进行粗提取。方法:从E.coli DH5 q中用碱提取质粒的方法提取质粒pEGFP-N舜口质粒pET-28a。然后用质粒DNA勺...
hygromicine gene and hsp70 terminator while the plasmid p Vectour libre only contains hsp70 promoter and terminator in order to be inserted by interesting genes.The specific gene primers were designed based on green fluoresent protein ( GFP) sequence,then GFP gene was obtained by PCR,and ...
Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila Proc Natl Acad Sci USA, 92 (1995), pp. 7036-7040 95350206 Google Scholar [16] K Wada, Y Wada, H Doi, F Ishibashi, T Gojobori, T Ikemura Codon usage tabulated from the GenBank genetic sequence data...
Green fluorescent protein (GFP) is a 27 kDa protein originally isolated from the jellyfishAequoreavictoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in...
Detection in living cells of Ca2+-dependent changes in thefluorescence emission of an indicator composed of two greenfluorescent protein variants linked by a calmodulin-bindingsequence.Anew class of fluorescent indicators.即使用由两种绿色荧光蛋白变体经钙调蛋白结合序列相连而构成的指示剂在活细胞中检测依赖Ca...
Image tubulin dynamics in live cells with CellLight Tubulin-GFP. This ready-to-use fluorescent protein-based reagent contains a tubulin targeting sequence fused to emerald GFP (emGFP) and utilizes the powerful BacMam delivery technology. To u