If you really have to needle-pass infected cells to liberate parasites, leave the plastic sheath on the needle and cut off its tip, using sturdy scissors, several millimeters before the tip of the actual needle. This can help to protect you from accidental sticks, and provides extra safety ...
Phi29 在本文,我们首先对滚环扩增中所用的phi29 DNA聚合酶链具有5’.3’聚合酶特性以及3’.5’外切酶校读功能。我们利用phi29 DNA聚合酶的3’-夕f、切酶活性对靶核酸片段上3’末端的非互补序列进行剪切,考 察不同长度的非互补序列对滚环扩增效率的影响,并成功地对含有G2677T/A核 万方数据 苷酸序列进行了...
no-cut right end (Blattner et al., 1977SDS sodium dodecyl sulfatesodium dodecyl sulfateThree new 位 phage cloning vectors have been constructed, Charons (Ch) 27, 28 and 30. Ch27 and Ch30, are suitable for cloning small and large DNA fragments, respectively, cut with BamHI, BglII, ...